Specificity of antitermination mechanisms
In: Journal of Molecular Biology, Jg. 222 (1991-11-01), S. 59-66
Online
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Zugriff:
Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at the end of an rrn operon, such as rrnB. We have tested the specificity with which the T1-T2 terminators override an antitermination mechanism, by placing the terminator cluster downstream from the nut and qut sites recognized by phage λ N and Q gene antiterminators, respectively. Measurement of downstream gene expression shows that RNA polymerase modified by either N or Q reads through the T1-T2 terminators quite efficiently. This supports the view that T1-T2 are not superterminators, and that the rrn antitermination mechanism may have a restricted terminator specificity.
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Specificity of antitermination mechanisms
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Autor/in / Beteiligte Person: | Das, Asis ; Peralta, Evangeline ; DeVito, Joseph ; Bhattacharya, Pramatha ; Ghosh, Balaram ; Grzadzielska, Elizabeth |
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Zeitschrift: | Journal of Molecular Biology, Jg. 222 (1991-11-01), S. 59-66 |
Veröffentlichung: | Elsevier BV, 1991 |
Medientyp: | unknown |
ISSN: | 0022-2836 (print) |
DOI: | 10.1016/0022-2836(91)90737-q |
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