NGHIÊN CỨU QUY TRÌNH TÁCH CHIẾT CAROTENOID TỔNG SỐ TỪ LÁ CÚC TẦN (PLUCHEA INDICA LESS.) THU HÁI TẠI THÁI NGUYÊN

Carotenoid are used in foods, cosmetics and medicine. Nowadays, the main material resources are extractions from plants. The purpose of this research is to evaluate single factors: drying temperature, ratios of solution/material (ether petroleum), ultrasonic time, the temperature of extraction, effect to the extraction process of total carotenoid from Pluchea indica (L.) Less. The experiments gave corresponding results: 60 °C, 10/1( ml/g), 5 minutes, 60 °C. The evaluation of single factors affecting extraction conditions indicates that the factors which affect strongly to extraction process including the ratios of solution/material, ultrasonic time, and temperature of extraction. Depending on the evaluation by Box- Behnken method, the optimal condition for total carotenoid extraction from Pluchea indica (L.) Less is defined with the ratio of solution/material is 10.98 (ml/g); ultrasonic time is 7.04 minutes; the temperature of extraction is 61.58 °C. The amount of total carotenoid is 5.21795 mg/100g. This result is highly compatible with the model.