Diagnosis of breast cancer recurrence using a microfluidic device featuring tethered cationic polymers
In: Applied Physics Letters, Jg. 99 (2011-07-04), S. 013701-13701
Online
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Zugriff:
In this study, we grafted pH-responsive poly(2-dimethylaminoethyl methacrylate) (PDMAEMA) onto a Si substrate as the medium in a microfluidic device to detect breast cancer recurrence DNA (bcrDNA584) and a control human genomic DNA (hgDNA528) at extremely low concentrations (down to 0.15 ng/μl). The quantities of these two DNAs obtained through the capture and release from tethered PDMAEMA brushes under pH tuning conditions were sufficient for them to be amplified recognizably, suggesting that this approach could be used in miniaturized lab-on-a-chip cartridges for rapid disease diagnosis.
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Diagnosis of breast cancer recurrence using a microfluidic device featuring tethered cationic polymers
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Autor/in / Beteiligte Person: | Chen, Jem-Kun ; Bai, Bing-Jun ; Chang, Feng-Chih |
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Zeitschrift: | Applied Physics Letters, Jg. 99 (2011-07-04), S. 013701-13701 |
Veröffentlichung: | AIP Publishing, 2011 |
Medientyp: | unknown |
ISSN: | 1077-3118 (print) ; 0003-6951 (print) |
DOI: | 10.1063/1.3608239 |
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