Acute Inactivation of the VHL Gene Contributes to Protective Effects of Ischemic Preconditioning in the Mouse Kidney
In: Nephron Experimental Nephrology, Jg. 110 (2008-10-27), S. e82- (9S.)
Online
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Zugriff:
Background/Aims: The von Hippel-Lindau (VHL) protein functions as an E3 ubiquitin ligase, controlling the stability of hypoxia-inducible factor (HIF). Preinduction of HIF-1α before pathological insult activates a self-defense mechanism and suppresses further aggravation of organ or cellular injury by ischemia. We investigated whether acute inactivation of the VHL gene might play a role in the response of mice to ischemic renal injury. Methods: We generated tamoxifen-inducible conditional VHL knockout (VHL-KO) mice to inactivate the VHL gene in an acute manner during renal ischemia-reperfusion injury (IRI) induced by bilateral clamping of kidney arteries. Renal IRI is characterized by renal dysfunction and tubular damage. Results: After the procedure of IRI, blood urea nitrogen (BUN) and creatinine (CRN) levels in control mice were significantly higher (BUN, 138.10 ± 13.03 mg/dl; CRN, 0.72 ± 0.16 mg/dl) than in VHL-KO mice (BUN, 52.12 ± 6.61 mg/dl; CRN, 0.24 ± 0.04 mg/dl; BUN: p < 0.05; CRN: p < 0.05). Histologically, tubular injury scores were higher in control mice than in VHL-KO mice (p < 0.05). Conclusion: We suggest that the acute inactivation of the VHL gene contributes to protective effects of ischemic preconditioning in renal tubules of the mouse.
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Acute Inactivation of the VHL Gene Contributes to Protective Effects of Ischemic Preconditioning in the Mouse Kidney
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Autor/in / Beteiligte Person: | Furihata, Mutsuo ; Sato, Takayuki ; Kurabayashi, Atsushi ; Shuin, Taro ; Schmidt, Laura S. ; Hong, Seung-Beom ; Iguchi, Mitsuko ; Kakinuma, Yoshihiko |
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Zeitschrift: | Nephron Experimental Nephrology, Jg. 110 (2008-10-27), S. e82- (9S.) |
Veröffentlichung: | S. Karger AG, 2008 |
Medientyp: | unknown |
ISSN: | 1660-2129 (print) |
DOI: | 10.1159/000166994 |
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