Kompartimentierung und Eigenschaften der MDH-Isoenzyme aus Wassermelonenkeimbl�ttern
In: Planta, Jg. 112 (1973), S. 137-148
Online
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Zugriff:
Five MDH-isoenzymes (I–V) from cotyledons of dark-grown water-melon seedlings older than 2 days can be identified by disc-electrophoresis. By isolating and fractionating cell organelles (10000 g fraction) by density gradient centrifugation (Fig. 1) the following compartmentation of the MDH-isoenzymes can be shown: the mitochondria contain isoenzyme III and the glyoxysomes preponderantly (if not exclusive) isoenzyme V (Fig. 2), whereas the isoenzymes I, II, and IV belong to the cytosol. The 5 MDH-isoenzymes differ in several properties, e.g. differential precipitation by ammonium sulfate (Fig. 4) or isoelectric point (Fig. 5). The glyoxysomal MDH is a relatively basic protein (isoelectric point at pH 8.7), whereas the isoelectric points of the other isoenzymes lie between pH 6.4 (IV) and pH 4.7 (I).
Titel: |
Kompartimentierung und Eigenschaften der MDH-Isoenzyme aus Wassermelonenkeimbl�ttern
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Autor/in / Beteiligte Person: | Hock, B. |
Link: | |
Zeitschrift: | Planta, Jg. 112 (1973), S. 137-148 |
Veröffentlichung: | Springer Science and Business Media LLC, 1973 |
Medientyp: | unknown |
ISSN: | 1432-2048 (print) ; 0032-0935 (print) |
DOI: | 10.1007/bf00388584 |
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