ACTIVITY OF CATHEPSIN H IN BREAST TUMORS AND ADJACENT TISSUES IN WOMEN WITH INFILTRATING DUCTAL BREAST CANCER
In: Odesa National University Herald. Biology, Jg. 20 (2015-11-28)
Online
unknown
Zugriff:
The cancer of mammary gland (CMG) occupies a leading place in the structure of oncologic morbidity of women, and most often there is an infiltrating-ductal form. For many types of tumours of man the increase of activity of proteolytic enzymes, that take part in an angiogenesis, prolyferation and innidiation, is shown. However the biological role of cathepsin of Н in neoplasm of human is studied not enough. A research aim consisted of study of activity of cathepsin of Н in tumour fabric of infiltrating-ductal form of CMG for development of additional biochemical methods of diagnostics. In a supernatant determined maintenance of albumen on a biuret method and activity of cathepsin of Н on the method of Bredshow. Activity of enzyme was expressed in mkm leucine appearing in the process of hydrolysis of oxytocinum, on the mg of albumen in mines at 37 °С. Statistical treatment of results of researches was conducted in accordance with U- by a criterion god Mann-Whitney. The results of researches testify to active voice of cathepsin of Н on the initial stages of progression mastoncuss that is accompanied by local destruction of intercellular matrice, that provides an invasion and innidiation of tumour cages. It is shown that a peak of met of infiltrating-ductal form of CMG is on 51-60, and lobular-infiltration form of CMG - on 61-70. It is set that maintenances of albumen in tumour fabric in 1,9 - 2,3 time higher, than maintenance of albumen is in fabric abutting upon a tumour. Maximal maintenance of albumen in fabric of tumour was set on ІІ and ІІ - the ІІІ stages of her development, in and further accompanied by the decline of maintenance of albumen. It is shown that relative activity of cathepsin of Н higher in tumour fabric of mammary gland, as compared to the unchanged bordering fabric regardless of the stage of development of tumour and maximal on І - ІІ, II - III of the stage. Maximal specific activity of enzyme is set on І - the ІІ stage of development of tumour. Progression of tumour is accompanied by a decline in her to specific activity of cathepsin of Н and in bordering healthy fabric on a background practically not changing maintenance of albumen. The method of determination of activity of cathepsin of Н it is expedient to use as additional research on the early stages of development of infiltrating-ductal CMG.
Titel: |
ACTIVITY OF CATHEPSIN H IN BREAST TUMORS AND ADJACENT TISSUES IN WOMEN WITH INFILTRATING DUCTAL BREAST CANCER
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Zeitschrift: | Odesa National University Herald. Biology, Jg. 20 (2015-11-28) |
Veröffentlichung: | Odesa I.I. Mechnikov National University, 2015 |
Medientyp: | unknown |
ISSN: | 2077-1746 (print) |
DOI: | 10.18524/2077-1746.2015.2(37).54910 |
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