Aqueous Extract of Moringa Oleifera Lam. Leaves Added to Freezing Extenders Damages Goat Sperm Membranes
In: Cryoletters, Jg. 44 (2023-05-01), S. 151-159
Online
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Zugriff:
BACKGROUND: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant . OBJECTIVE: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency. MATERIALS AND METHODS: Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed. RESULTS: With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (ρ < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (ρ < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner. CONCLUSION: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner.
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Aqueous Extract of Moringa Oleifera Lam. Leaves Added to Freezing Extenders Damages Goat Sperm Membranes
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Autor/in / Beteiligte Person: | Desirée Coelho de Mello Seal ; Millena Maria Monteiro ; Lúcia Cristina Pereira Arruda ; Jerônimo Hugo de Souza ; Robson Raion de Vasconcelos Alves ; Luiz Alberto Lira Soares ; Thiago Henrique Napoleão ; Patrícia Maria Guedes Paiva ; Lucas Eduardo Bezerra de Lima ; Regina Célia Bressan Queiroz de Figueiredo ; Maria Madalena Pessoa Guerra |
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Zeitschrift: | Cryoletters, Jg. 44 (2023-05-01), S. 151-159 |
Veröffentlichung: | CryoLetters Limited Liability Partnership, 2023 |
Medientyp: | unknown |
ISSN: | 0143-2044 (print) |
DOI: | 10.54680/fr23310110712 |
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