Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scaffold fabricated by 3D printing
In: Bio-Design and Manufacturing, Jg. 3 (2020-07-18), S. 396-409
Online
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Zugriff:
Bone is known to have a natural function to heal itself. However, if the bone damage is beyond a critical degree, intervention such as bone grafting may be imperative. In this work, the fabrication of a novel bone scaffold composed of natural bone components and polycaprolactone (PCL) using 3D printing is put forward. α1, 3-galactosyltransferase deficient pigs were used as the donor source of a xenograft. Decellularized porcine bone (DCB) with attenuated immunogenicity was used as the natural component of the scaffold with the aim to promote bone regeneration. The 3D printed DCB-PCL scaffolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive strength. The biological properties of the DCB-PCL scaffolds were evaluated by studying cell adhesion, viability, alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem cells. The in vitro results demonstrated that the DCB-PCL scaffolds exhibit an enhanced performance in promoting bone differentiation, which is correlated to the DCB content. Furthermore, critical-sized cranial rat defects were used to assess the effect of DCB-PCL scaffolds on bone regeneration in vivo. The results confirm that in comparison with PCL scaffolds, the DCB-PCL scaffolds can significantly improve new bone formation in cranial defects. Thus, the proposed 3D printed DCB-PCL scaffolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects.
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Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scaffold fabricated by 3D printing
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Autor/in / Beteiligte Person: | Qiongxi, Pan ; Douroumis, Dennis ; Wang, Yingying ; Wang, Yili ; Li, Huaqiong ; Mao, Cong ; Lay Poh Tan ; Wen, Feng ; Gao, Chenyuan ; Wang, Quan ; Economidou, Sophia N. |
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Zeitschrift: | Bio-Design and Manufacturing, Jg. 3 (2020-07-18), S. 396-409 |
Veröffentlichung: | Springer Science and Business Media LLC, 2020 |
Medientyp: | unknown |
ISSN: | 2522-8552 (print) ; 2096-5524 (print) |
DOI: | 10.1007/s42242-020-00086-4 |
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