Increased NOX1 and DOUX2 Expression in the Colonic Mucosa of Patients with Chronic Functional Constipation
Research Square Platform LLC, 2021
Online
unknown
Zugriff:
Aim To determine whether oxidative stress and inflammation are associated with constipation by examining the expression of the main producers of reactive oxygen species, NADPH oxidases, and pro-inflammatory cytokines in the colon of patients with chronic functional constipation. Methods The colonic biopsies were collected from 32 patients with chronic functional constipation and 30 healthy subjects who underwent colonoscopy. Colonic mucosal histology was observed. IL-1β, IL-6, IL-8 mRNA, and four members of NADPH oxidase (NOX1, NOX2, DOUX2 and NOX4) protein and mRNA were assessed by immunohistochemistry, western blotting and RT-PCR. Results The tissues from both patients and healthy subjects showed normal histological structure without increase of inflammatory cells. NOX1 protein and mRNA levels were significantly increased compared to controls (P
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Increased NOX1 and DOUX2 Expression in the Colonic Mucosa of Patients with Chronic Functional Constipation
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Autor/in / Beteiligte Person: | Xue, Mei ; Chu, Fong-Fong ; Wei, Xiuqin ; Jiang, Shujing ; Gao, Qiang ; Ma, Chao ; Zhang, Mengqiao ; Cao, Lei ; Zheng, Yufeng ; Chen, Pan ; Gao, Lei ; Kang, Chunbo |
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Veröffentlichung: | Research Square Platform LLC, 2021 |
Medientyp: | unknown |
DOI: | 10.21203/rs.3.rs-643716/v1 |
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