AB0152 The differential diagnostic specificities of purine enzymes activity in rheumatoid arthritis and gout

Background The clinical picture of gouty arthritis (GA) and rheumatoid arthritis (RA) can be quite often much alike, and the differential diagnosis of these diseases is rather difficult. The GA and RA patients often have hyperuricemia, which is indicative of purine metabolism breaches in patients with these diseases. Objectives To study differential diagnostic specificity of Purine enzymes activity in Rheumatoid arthritis and Gout. Methods We observed 71 RA patients. The mean age of the patients ? 43.2 ± 3.6 years. The mean duration of the disease – 11.9 ± 3.6 years. I degree of RA activity was determined in 17 (23.94%), II ? in 44 (61.97%), III ? in 10 (14.09%) observations. 34 Gout patients were examined. The mean age of the patients ? 48.2 ± 2.2 years. The mean duration of the disease 8.8 ± 1.3 years. The intermittent form of Gout was determined in 21 (61.8%), the chronic form ? in 13 (38.2%) observations. Adenosin deaminase (ADA), AMP-desaminase (AMPDA), Xanthine dehydrogenase (XDG), Xanthine oxidase (XO) activities were measured with spectrophotometer, ADA, XDG isoenzymes were examined with method of electrophoresis in agarose gel and obtained enzymograms were evaluated at densitometry. Results XDG activity was more high, XO/XDG activities ratio was less in Gout patients in comparison with RA patients (I degree of activity). ADA-2 isoenzymes were more in chronic form of Gout than in RA. We revealed the simultaneous increase of XO, XDG activities in Gout (XO/XDG activities ratio ? 0.6–0.8), but increased XO activity in RA was accompanied by normal or lowered XDG activity (XO/XDG activities ratio ? more 1.0). Conclusion XO, XDG activities changes is more essential in differential diagnostics of RA and Gout among studied enzymatic indices.