Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol–Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins
In: Methods in Molecular Biology ISBN: 9781493940127; (2016)
Online
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Zugriff:
Capillary isoelectric focusing (CIEF) is a high-resolution technique for the separation of ampholytes, such as proteins, according to their isoelectric point. CIEF coupled online with MS is regarded as a promising alternative to 2-D PAGE for fast proteome analysis with high-resolving capabilities and enhanced structural information without the drawbacks of conventional slab-gel electrophoresis. However, online coupling has been rarely described, as it presents some difficulties. A new methodology for the online coupling of CIEF with electrospray ionization mass spectrometry (ESI-MS) has been developed in glycerol-water media. This new integrated methodology provides a mean for the characterization of a large number of hydrophilic and hydrophobic proteins.
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Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol–Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins
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Autor/in / Beteiligte Person: | Varenne, Anne ; d’Orlyé, Fanny ; Mokaddem, Meriem |
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Quelle: | Methods in Molecular Biology ISBN: 9781493940127; (2016) |
Veröffentlichung: | Springer New York, 2016 |
Medientyp: | unknown |
ISBN: | 978-1-4939-4012-7 (print) |
DOI: | 10.1007/978-1-4939-4014-1_5 |
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