AB0024 Activities of purine metabolism enzymes and antioxidant system in rheumatoid arthritis patients

Background The results of Antioxidant system researches of blood at Rheumatoid arthritis are inconsistent and not enough attention was given to Purine metabolism questions. Objectives The improvement of Rheumatoid arthritis diagnostics with use of the enzymatic tests. Methods 71 RA patients and 30 healthy controls were under observation. Adenosin deaminase (ADA), AMP-desaminase (AMPDA), Guanin desaminase (Guanase), Xanthine oxidase (XO), Xanthine dehydrogenase (XDG) activities were determined in blood serum; Glutation peroxidase (GP), Glutation reductase (GR), Superoxid dismutase (SOD) activities ? in plasma (pl.) and erythrocytes (er.). ADA, XDG, SOD isoenzymes were determined also. The enzymatic indices were defined in the process of treatment thrice and were analysed depending on clinical specificity of the disease. Results Increased ADA, Guanase, GRpl., GPer., XO, XDG, SOD activities, decreased AMPDA, GRer., GPpl. activities, increase ADA-2, XDG-2, SOD-1 isoenzymes were observed in RA patients (I degree of activity) in comparison with healthy controls. The increase of the pathological process activity was accompanied with the increase of Guanase, GRpl., XO, SODpl. activities, ADA-2, XDG-2, SOD-1 isoenzymes and the decrease of ADA, XDG, GRer. activities. RA patients with system defeats have more increased AMPDA, Guanase, XO activities, ADA-2, XDG-2 isoenzymes, but less ADA, XDG activities in comparison with patients, which had arthritis form of RA. The system defeates were characterised with the decrease of SOD, GPer. activities and the increase of GPpl. activity; patients with the arthritis form of RA had contrary changes. We observed more increase AMPDA, Guanase, XO, ADA-2 activities and less ADA activity in fast progressing RA in comparison with slow progressing. Conclusion The enzymatic indices essentially depended on clinical specificity of the disease and can be used as additional diagnostic criterions in RA.