Partial refinement of fungal chitinase (Beauveria bassiana) with multistage membrane filtration

Microscopic organisms play a vital role in the field of green technology. Among the genera fungi, the group of entomopathogens includes Beauveria bassiana, which has been found to have multifunctional applications. In this present research, we have attempted to partially purify chitinase from two strains of Beauveria bassiana (F881 and F911), each cultured in minimal media with 1% (w/v) colloidal chitin as well as in Sabouraud's dextrose media with 1% (w/v) colloidal chitin. The purification process was done with a setup of ceramic membranes, which comprises two stages, ultrafiltration (50 nm pore size membrane) and nanofiltration (MWCO 5 kDa). Furthermore, with the analysis of yield of enzymatically hydrolyzed NAG (N-acetyl glucosamine), resistance and fouling of the membrane were also studied.