Biochemical Markers for Carica papaya, C. cauliflora, and Plants from Somatic Embryos of Their Hybrid
In: Journal of the American Society for Horticultural Science, Jg. 109 (1984-03-01), S. 213-218
Online
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Zugriff:
Isozyme markers for glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), peroxidase (PER), and malate dehydrogenase (MDH) were identified for Carica papaya L. and the related but sexually incompatible C. cauliflora Jacq. These markers were used to determine the nature of somatic embryos derived from papaya ovules cultured on modified Murashige and Skoog (MS) medium 65 days after controlled pollination with C. cauliflora. Zymograms of plantlets from somatic embryos contained bands specific to either C. papaya or C. cauliflora (PER, GOT) and a unique band not present in the zymogram of either species (PER). Zymograms of somatic embryo-derived plantlets were distinctively different from those of either of the Carica species for all the enzyme systems examined. Evidence from isozyme markers indicates that somatic embryos produced from cultured papaya ovules following pollination with C. cauliflora may be hybrids. The isozyme banding patterns of 60 plantlets derived from somatic embryos from the same ovule were very uniform and suggest genetic uniformity among the regenerated plantlets.
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Biochemical Markers for Carica papaya, C. cauliflora, and Plants from Somatic Embryos of Their Hybrid
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Autor/in / Beteiligte Person: | Moore, G. A. ; Litz, R. E. |
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Zeitschrift: | Journal of the American Society for Horticultural Science, Jg. 109 (1984-03-01), S. 213-218 |
Veröffentlichung: | American Society for Horticultural Science, 1984 |
Medientyp: | unknown |
ISSN: | 2327-9788 (print) ; 0003-1062 (print) |
DOI: | 10.21273/jashs.109.2.213 |
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