Preconditioning with 1,25-Dihydroxyvitamin D3 Protects against Subsequent Ischemia-Reperfusion Injury in the Rat Kidney
In: Nephron Experimental Nephrology, Jg. 100 (2005-03-18), S. e85- (10S.)
Online
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Zugriff:
Background/Aim: Induction of heat shock protein 70 (HSP70) is important in the tolerance of subsequent ischemia-reperfusion (I/R) injury. The aim of this study was to evaluate the effect of HSP70 induction by 1,25-dihydroxyvitamin D3 (VD3) on subsequent I/R injury in rats. Methods: HSP70 was induced in Sprague-Dawley rats by VD3 treatment for 7 days, and the effect of VD3 pretreatment on subsequent I/R injury was evaluated in terms of renal function, tubular necrosis score, tumor necrosis factor alpha mRNA expression, mitogen-activated protein kinase expression, and proliferating cell nuclear antigen expression. Results: VD3 treatment increased HSP70 expression which was localized to renal tubular cells in the outer medulla. Pretreatment with VD3 before I/R injury resulted in (1) decreased blood urea nitrogen and serum creatinine levels; (2) decreased tubular cell necrosis; (3) increased tubular cell proliferation as determined by proliferating cell nuclear antigen expression; (4) decreased tumor necrosis factor alpha mRNA expression, and (5) increased extracellular signal regulated protein kinase and decreased c-Jun N-terminal kinase expression. Conclusion: Our study demonstrates that VD3 is a nontoxic inducer of HSP70 and exerts a protective effect against subsequent I/R injury.
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Preconditioning with 1,25-Dihydroxyvitamin D3 Protects against Subsequent Ischemia-Reperfusion Injury in the Rat Kidney
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Autor/in / Beteiligte Person: | Sun Woo Lim ; Yong Soo Kim ; Young Ok Kim ; Kim, Jin ; Bun Soon Choi ; Bo Kyung Sun ; Li, Can ; Jung Sim Kim ; Chul Woo Yang ; Byung Kee Bang |
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Zeitschrift: | Nephron Experimental Nephrology, Jg. 100 (2005-03-18), S. e85- (10S.) |
Veröffentlichung: | S. Karger AG, 2005 |
Medientyp: | unknown |
ISSN: | 1660-2129 (print) |
DOI: | 10.1159/000084574 |
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