BIOCHEMICAL PROPERTIES AND DEVELOPMENT EXPRESSION OF GENETICALLY DETERMINED MALATE DEHYDROGENASE ISOZYMES IN MAIZE
Elsevier, 1975
Online
unknown
Zugriff:
Genetic and biochemical studies suggest that both soluble and mitochondrial malate dehydrogenase (MDH) isozymes in maize are genetically determined, and are not different conformational forms derived from the same primary structure. Results of the genetic analysis also show that the five commonly observed mitchondrial malate dehydrogenase (m-MDH) isozymes are under the control of two groups of loci residing on two different chromosomes. Biochemical properties for each of the soluble and mitochondrial MDH isozymes in the highly inbred strain W64A were examined. Not only the soluble malate dehydrogenases(s-MDHs) and m-MDHs differ in most of their physical and kinetic properties, the MDH isozymes within the same subcellular location may also differ significantly in several of these properties examined. Developmental expression of the multiple genes controlling MDH isozymes has been studied in the young maize seedlings. It is found that both s-MDHs and m-MDHs in the scutellum of developing maize seedlings are de novo synthesized. The increase of both classes of MDHs are inhibited by cycloheximide, but not by chloramphenicol. These results indicate that the nuclear gene controlled mitochondrial MDH isozymes are synthesized in the cytoplasm and then become associated with the mitochondria.
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BIOCHEMICAL PROPERTIES AND DEVELOPMENT EXPRESSION OF GENETICALLY DETERMINED MALATE DEHYDROGENASE ISOZYMES IN MAIZE
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Autor/in / Beteiligte Person: | Yang, Ning-Sun |
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Veröffentlichung: | Elsevier, 1975 |
Medientyp: | unknown |
DOI: | 10.1016/b978-0-12-472703-8.50020-2 |
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