PI-Based Separation of Tissue Proteins Realized with the Use of Two-Dimensional Gel Electrophoresis or Alternatively Capillary Isoelectric Focusing
In: The Open Proteomics Journal, Jg. 5 (2012-10-19), S. 22-28
Online
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Zugriff:
A two-dimensional gel electrophoresis (2D-GE) and capillary isoelectric focusing (cIEF) were performed sub- sequently to analyze protein mixtures from laboratory animal tissues (liver, muscle, and mammary gland). Both tech- niques were compared and evaluated. It was found that cIEF can be a valuable alternative in comparison to 2D-GE. Espe- cially considering the goals associated with the determination of far the separated proteins, cIEF possesses perspective analytical advantages.
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PI-Based Separation of Tissue Proteins Realized with the Use of Two-Dimensional Gel Electrophoresis or Alternatively Capillary Isoelectric Focusing
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Autor/in / Beteiligte Person: | Olędzka, Ilona ; Paradziej-Lukowicz, Jolanta ; Kowalski, Piotr ; Baczek, Tomasz ; Grzenkowicz-Wydra, Jolanta ; Wlodarski, K. |
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Zeitschrift: | The Open Proteomics Journal, Jg. 5 (2012-10-19), S. 22-28 |
Veröffentlichung: | Bentham Science Publishers Ltd., 2012 |
Medientyp: | unknown |
ISSN: | 1875-0397 (print) |
DOI: | 10.2174/1875039701205010022 |
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