Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone
In: Journal of Biological Chemistry, Jg. 268 (1993-10-01), S. 20733-20736
Online
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Zugriff:
Previous attempts to express glycosylphosphatidylinositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.
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Correction of the class H defect in glycosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone
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Autor/in / Beteiligte Person: | Hui Ming Chang ; Rollins, Carl T. ; Edward T.H. Yeh ; Waneck, Gerald L. ; Kamitani, Tetsu |
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Zeitschrift: | Journal of Biological Chemistry, Jg. 268 (1993-10-01), S. 20733-20736 |
Veröffentlichung: | Elsevier BV, 1993 |
Medientyp: | unknown |
ISSN: | 0021-9258 (print) |
DOI: | 10.1016/s0021-9258(19)36842-5 |
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