Specifically alkylated DNA fragments. Synthesis and Physical Characterization of D[cGC(o2Me)GCG]and d[cGT(O6Me) GCG]
In: Nucleic Acids Research, Jg. 11 (1983), S. 3393-3404
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Zugriff:
Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40° lower than the unmodified parent compund, d(CG)3. Moreover, the presence of the (O6Me)G appears to inhibit the B→Z transition, as determined by CD spectroscopy.
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Specifically alkylated DNA fragments. Synthesis and Physical Characterization of D[cGC(o2Me)GCG]and d[cGT(O6Me) GCG]
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Autor/in / Beteiligte Person: | Kuzmich, Sandra ; Jones, Roger A. ; Marky, Luis A. |
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Zeitschrift: | Nucleic Acids Research, Jg. 11 (1983), S. 3393-3404 |
Veröffentlichung: | Oxford University Press (OUP), 1983 |
Medientyp: | unknown |
ISSN: | 1362-4962 (print) ; 0305-1048 (print) |
DOI: | 10.1093/nar/11.10.3393 |
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