Ribosomal RNA operon anti-termination
In: Journal of Molecular Biology, Jg. 209 (1989-10-01), S. 345-358
Online
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Zugriff:
All Escherichia coli rrn operons show a common motif in which anti-terminator box B-box A sequences occur twice, first in the leader and again in the 16 S–23 S spacer. In this study we have analyzed several aspects of rrn anti-termination by leader and spacer anti-terminator sequences. Using DNA synthesis and a plasmid test system, we incorporated random changes into the leader anti-terminator region and examined these mutations for their ability to read through a strong terminator. We also examined anti-termination by synthetic box A and by rrn spacer region sequences. Information derived from these experiments was used to search the rrn sequences of other micro-organisms for possible anti-termination features. Our principal conclusions were that: (1) box A was sufficient for terminator readthrough; (2) we could show no positive requirement for box B in our test system; (3) many of the negative anti-terminator mutations caused a promoter up-effect in the absence of a terminator; (4) the search of rrn operons from other micro-organisms revealed that anti-terminator-like box B-box A sequences exist in leader and spacer regions of both eubacteria and archaebacteria. The frequent occurrence of this pattern suggested that the E. coli rrn anti-termination motif is widespread in nature and has been conserved in microbial evolution.
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Ribosomal RNA operon anti-termination
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Autor/in / Beteiligte Person: | Squires, Catherine L. ; Squires, Craig ; Berg, Karen L. |
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Zeitschrift: | Journal of Molecular Biology, Jg. 209 (1989-10-01), S. 345-358 |
Veröffentlichung: | Elsevier BV, 1989 |
Medientyp: | unknown |
ISSN: | 0022-2836 (print) |
DOI: | 10.1016/0022-2836(89)90002-8 |
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