miR-574-5p regulates milk synthesis and cell survival activities via DAG-DGKs-PA pathway in GMECs
Research Square Platform LLC, 2020
Online
unknown
Zugriff:
miRNA-regulated gene expression is momentous for mammary development and lactation performance. Prevenient work has revealed that miR-574-5p is capable of functioning on goat mammary epithelial cells, but the potential molecular mechanism remains to be further understood. According to transcriptome sequencing results, we focused on DGKI and DGKH, which both were down-regulated differentially expressed unigenes by miR-574-5p. miR-574-5p objected seed sequences of the DGKI 3’UTR and interdicted mRNA and protein levels of DGKI and DGKH, correspondingly. It’s known that diacylglycerol kinases phosphorylate diacylglycerol to produce phosphatidic acid then regulate a range of biological processes. Detection kits showed that DGKI and DGKH induced diglycerides conversion to phosphatidic acid, and DGKI facilitated triglycerides and β-casein production while DGKH only acted as a promoter of triglycerides. DGKI inhibited cell apoptosis and induced cell proliferation, and co-expression of miR-574-5p with DGKI proved an adiaphorous impact. However, DGKH had an anti-proliferative and pro-apoptotic effects on GMECs. Additionally, DGKI motivated both AKT-mTOR and Raf-1-ERK pathways while DGKH showed negative impacts on AKT-mTOR, Raf-1-ERK and IKK-NFKB pathways in GMECs. It suggested that miR-574-5p not only inactivated PA-mTOR pathway and contributed to degressive secretion of triglycerides and β-casein via depletion of DGKI, but also arrested cell proliferation and enhanced cell apoptosis by inhibiting DGKI-PA-ERK pathway. All these established a farther regulatory mechanism of miR-574-5p in milk synthesis and mammary development.
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miR-574-5p regulates milk synthesis and cell survival activities via DAG-DGKs-PA pathway in GMECs
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Autor/in / Beteiligte Person: | Wang, Jiangang ; Cao, Binyun ; Zhang, Meng ; An, Xiaopeng ; Dan, Guo ; Liu, Yuhan |
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Veröffentlichung: | Research Square Platform LLC, 2020 |
Medientyp: | unknown |
DOI: | 10.21203/rs.2.21864/v1 |
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