Use of Porter–Silber and Schiff Reagents as Spot Tests for Steroids applied on Paper and their Application to the Study of Rat Adrenal Lipids
In: Nature, Jg. 184 (1959-09-01), S. BA67- (2S.)
Online
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Zugriff:
THE formation of phenylhydrazones with an absorption maximum at 410 mµ by the method of Porter and Silber characterizes steroids with a dihydroxyacetone side-chain. These authors recently reported that the reaction also occurred with the aldehydes of corticosterone and of 11-dehydrocorti-costerone and that with the latter compounds the phenylhydrazone developed at a more rapid rate1. The specificity and difference in speed of the reaction are also evident when it is carried out on paper. A bright yellow colour develops when a region containing a minimum of 2 µgm./cm.2 of the chromogen is passed through the Porter–Silber reagent (25 ml. water, 41 ml. conc. sulphuric acid, 34 ml. ethyl alcohol and 43 mgm. phenylhydrazine hydrochloride). The colour appears instantly with C-21 aldehydes and their acetates, and in 1–2 hr. with steroids containing the dihydroxyacetone side-chain and their acetates. It is stable for days provided the paper is not rinsed or warmed, and under these conditions the paper does not char. Corticosterone, 17-hydroxyprogesterone and other steroids tested gave no colour at a concentration of 25 µgm./cm.2.
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Use of Porter–Silber and Schiff Reagents as Spot Tests for Steroids applied on Paper and their Application to the Study of Rat Adrenal Lipids
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Autor/in / Beteiligte Person: | Birmingham, Marion K. |
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Zeitschrift: | Nature, Jg. 184 (1959-09-01), S. BA67- (2S.) |
Veröffentlichung: | Springer Science and Business Media LLC, 1959 |
Medientyp: | unknown |
ISSN: | 1476-4687 (print) ; 0028-0836 (print) |
DOI: | 10.1038/184067a0b |
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