Cytochemical analysis of human T cell leukaemia virus 1 LTR-regulated β-galactosidase gene expression using a novel integrated cell system
In: Journal of Virological Methods, Jg. 45 (1993-12-01), S. 161-167
Online
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Zugriff:
To develop a reporter system to study the response of an integrated retroviral LTR and cellular and viral events which influence transcription, the 5' LTR of HTLV-1 was coupled to the Escherichia coli β-galactosidase gene ( lacZ ). This construct was assembled within a vector containing the neomycin resistance gene controlled by the SV40 promoter, and introduced into HeLa cells. Expression from the LTR in one clone was upregulated by positive regulators of HTLV-1 expression, including 12- O -tetradecanoylphorbol-13-acetate (TPA) and the HTLV-1 transregulatory protein (tax), as has been previously reported using transient transfection assays. This method proved to be a rapid and reproducible assay for the measurement of integrated viral LTR activation in a single cell system.
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Cytochemical analysis of human T cell leukaemia virus 1 LTR-regulated β-galactosidase gene expression using a novel integrated cell system
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Autor/in / Beteiligte Person: | Derse, David ; Heeney, Jonathan L. ; Anthonius G.M. Haaksma ; Goudsmit, Jaap ; Karen F.T. Copeland |
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Zeitschrift: | Journal of Virological Methods, Jg. 45 (1993-12-01), S. 161-167 |
Veröffentlichung: | Elsevier BV, 1993 |
Medientyp: | unknown |
ISSN: | 0166-0934 (print) |
DOI: | 10.1016/0166-0934(93)90100-6 |
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