Synthesis of CEG-AgNPs as a Promising MMPs/COX-2/Bcl-2 Signaling Pathway Modulator in BaP-Induced Lung Carcinoma
In: Asian Journal of Chemistry, Jg. 34 (2022), S. 289-296
Online
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Zugriff:
Cucurbitacins are a class of highly oxidized tetracyclic triterpenoids. It’s hydrophobic properties and poor solubility in water, polymeric micellar systems exhibited improved antitumor efficacy because of a better solubilization and targeting after local and/or systemic administration. The aim of the present work was to evaluate the anticancer activity of CEG-AgNPs against benzo[a]pyren (BaP)-induced lung carcinoma. CEG-AgNPs was prepared, characterized and evaluated for its cytotoxic activity against A549 lung carcinoma cell line. Also, the anticancer activity of CEG-AgNPs (70.25 mg/kg) against BaP-induced lung carcinoma was evaluated in vivo, using 30 adult mice for 43 days. IC50 of CEG-AgNPs against A549 lung carcinoma cell line were approximately 94.47 μg/mL. Administration of BaP (50 mg/kg b.w.) to mice induced lung carcinoma with a significant increase in lung MMP-2, MMP-9, MMP-12, MDA, IL-6 and NF-κB as well as significant decreased in lung CAT, GPx and GSH level. Also, treatment with BaP produced significant increase in lung VEGF-C, COX-2 and Bcl-2 gene expression as compared to control group. Daily oral administration of CEG-AgNPs to mice treated with BaP showed a significant protection against-induced increase in lung MMP-2, MMP-9, MMP-12, MDA, IL-6 and NF-κB levels. The treatment also resulted in a significant increase in lung CAT, GPx and GSH level. In addition, the CEG-AgNPs could inhibit lung VEGF-C, COX-2 and Bcl-2 gene expression as compared to BaP treated mice. The histological and MRI examination showed that a significant normalization has been observed through in CEG-AgNPs treated mice. The biochemical, histological and MRI results showed that CEG-AgNPs have potent anticancer activity against BaP-induced lung carcinoma through modulating multiple cellular behaviours and signaling pathways leading to the suppression of adaptive immune responses.
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Synthesis of CEG-AgNPs as a Promising MMPs/COX-2/Bcl-2 Signaling Pathway Modulator in BaP-Induced Lung Carcinoma
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Autor/in / Beteiligte Person: | Emara, Ahmed A. ; Darwesh, Ahmed M. ; Mostafa, Mohamed A. ; Ahmed, Ahmed A. ; Rashad, Khaled W. ; Said, Abdullah M. ; Elsebaay, Abdelrahman E. ; Nasser, Khaled M. ; Amin, Abdelrehem S. ; Gamil, Mohamed S. ; Mohamed, Foad H. ; Ahmed, Mohamed K. ; Alshrief, Diana A. ; Hassan, Mohammed E. ; Nassar, Zahraa ; El-Ella, Amr A. ; Abdel-Maksoud, Mostafa A. ; Hussein, Mohammed A. |
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Zeitschrift: | Asian Journal of Chemistry, Jg. 34 (2022), S. 289-296 |
Veröffentlichung: | Asian Journal of Chemistry, 2022 |
Medientyp: | unknown |
ISSN: | 0975-427X (print) ; 0970-7077 (print) |
DOI: | 10.14233/ajchem.2022.23456 |
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