Coumarin-derived Cu(2+)-selective fluorescence sensor: synthesis, mechanisms, and applications in living cells
In: Journal of the American Chemical Society, Jg. 131 (2009-02-05), Heft 5
Online
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Zugriff:
A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu(2+) over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu(2+) ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu(2+) in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu(2+).
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Coumarin-derived Cu(2+)-selective fluorescence sensor: synthesis, mechanisms, and applications in living cells
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Autor/in / Beteiligte Person: | Jong Wan Kim ; Jong Seung Kim ; Hyo Sung Jung ; Yan, Shihai ; Jae I I Kim ; Jung Hwa Lee ; Joo, Taiha ; Chang Seop Hong ; Jin Yong Lee ; Pil Seung Kwon ; Jeong Won Lee |
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Zeitschrift: | Journal of the American Chemical Society, Jg. 131 (2009-02-05), Heft 5 |
Veröffentlichung: | 2009 |
Medientyp: | unknown |
ISSN: | 1520-5126 (print) |
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