Dynamics and genetic diversity of Haemophilus influenzae carriage among French pilgrims during the 2018 Hajj: A prospective cohort survey
In: TRAVEL MEDICINE AND INFECTIOUS DISEASE TRAVEL MEDICINE AND INFECTIOUS DISEASE, 2020, 38, ⟨10.1016/j.tmaid.2020.101883⟩; (2020)
Online
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Zugriff:
International audience; Background: To investigate the genetic diversity of Haemophilus influenzae positive samples among French pilgrims attending the 2018 Hajj pilgrimage. Method: After screening by qPCR, multilocus sequence typing was performed for all H. influenzae-positive samples. The following housekeeping genes were amplified and assigned: adk, atpG, frdB, fucK, mdh, pgi and recA. Results: 121 pilgrims were included. H. influenzae was positive in 35.5% pre-Hajj samples, 12.4% at day five post-arrival, 15.7% at day 12 post-arrival, and 43.0% post-Hajj. Of the 129 positive swabs for H. influenzae, only one sample at D12 was negative for all seven genes amplified by standard PCR. The adk, atpG, frdB, mdh, pgi, recA and fucK genes were positive in 123, 107, 122, 70, 127, 118 and 69 samples, respectively. One sequence of atpG and two of recA genes were not possible to assign. None of the sequences of fucK gene was successfully obtained. Consequently, a complete sequence type characterisation was not possible. Of the 128 obtained strains, 111 had distinct patterns of alleles. Conclusion: H. influenzae genotypes acquired were completely different from those present at pre-Hajj. We observed a great biodiversity and a lack of clonality of H. influenzae among French pilgrims during the 2018 Hajj. Further studies aiming at studying the genome of Hajj-acquired H. influenzae isolates are needed to define the clinical burden of H. influenzae infection during Hajj and to evaluate the potential interest of vaccination in Hajj pilgrims.
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Dynamics and genetic diversity of Haemophilus influenzae carriage among French pilgrims during the 2018 Hajj: A prospective cohort survey
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Autor/in / Beteiligte Person: | Tran Duc Anh Ly ; Raoult, Didier ; Belhouchat, Khadidja ; Yezli, Saber ; Vincent Pommier de Santi ; Alotaibi, Badriah ; Kamel Larbi Chaht ; Parola, Philippe ; Gautret, Philippe ; Van Thuan Hoang ; Dao, Thi-Loi ; Fournier, Pierre-Edouard ; Vecteurs - Infections tropicales et méditerranéennes (VITROME) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA) ; Ministry of Health [Riyadh] ; Microbes évolution phylogénie et infections (MEPHI) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) ; Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille) ; Service de Santé des Armées ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA) |
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Quelle: | TRAVEL MEDICINE AND INFECTIOUS DISEASE TRAVEL MEDICINE AND INFECTIOUS DISEASE, 2020, 38, ⟨10.1016/j.tmaid.2020.101883⟩; (2020) |
Veröffentlichung: | HAL CCSD, 2020 |
Medientyp: | unknown |
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