Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China
In: Journal of Medical Microbiology, Jg. 64 (2015-08-01), S. 910-915
Online
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Zugriff:
The cfr gene was detected in 14 meticillin-susceptible Staphylococcus aureus isolates recovered from outpatients with community-onset infections in a county hospital in China. The MIC of linezolid was 4 μg ml- 1 in eight isolates and 2 μg ml- 1 in six isolates. All isolates were susceptible to vancomycin and teicoplanin, but had elevated MICs for penicillin (0.5-128 μg ml- 1), chloramphenicol (2-32 μg ml- 1), clindamycin (0.5-128 μg ml- 1) and erythromycin (4-128 μg ml- 1). Nine isolates had mutations on domain V of 23S rRNA and/or the ribosomal L proteins that were not located close to the linezolid-binding pocket. Southern blotting experiments demonstrated that the cfr genes in all 14 isolates resided on plasmids. Sequence analysis of the 5.6 kb cfr-carrying plasmid segment revealed 99 % identity to the corresponding sequences in plasmid pSS-01 from animal staphylococci and plasmid pRM-01 from human staphylococci. Five isolates belonged to sequence type (ST)188 and three to ST965; the two ST types were previously reported in isolates of animal origin in some areas of China. These results indicate that the cfr-carrying plasmids in this study are likely of animal origin. The present study shows that cfr-harbouring S. aureus isolates have emerged in some areas of China and that cfr-carrying isolates may be transmitted between animals and humans.
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Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China
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Autor/in / Beteiligte Person: | Xiao, Yonghong ; Shen, Ping ; Li, Lanjuan ; Li, Sujuan ; Zhao, Lina ; Ji, Jinru ; Zheng, Beiwen ; Lv, Jifang |
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Zeitschrift: | Journal of Medical Microbiology, Jg. 64 (2015-08-01), S. 910-915 |
Veröffentlichung: | Microbiology Society, 2015 |
Medientyp: | unknown |
ISSN: | 1473-5644 (print) ; 0022-2615 (print) |
DOI: | 10.1099/jmm.0.000096 |
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