Dieckol induces cell cycle arrest by down-regulating CDK2/cyclin E in response to p21/p53 activation in human tracheal fibroblasts
In: Cell biochemistry and functionREFERENCES, Jg. 40 (2021-09-11), Heft 1
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Zugriff:
The phlorotannin derivative dieckol isolated from Ecklonia cava has been shown to exhibit anti-inflammatory, anti-bacterial, anti-oxidative anti-adipogenic and anti-stenosis activity. However, the role of dieckol in cyclin-dependent kinase 2 (CDK2)/cyclin E signalling, which regulates fibrosis development, has not yet been determined. In this study, we report that dieckol-suppressed cell proliferation through the cell cycle arrest of Hs680.Tr human tracheal fibroblasts. Following consecutive purification, dieckol was identified as a potent bioactive compound. The results showed that dieckol had significant anti-proliferative activity against Hs680.Tr human tracheal fibroblastsWestern blotting analysis also found that dieckol dose-dependently induced the cell cycle arrest of Hs680.Tr fibroblasts in the G0/G1 phase, accompanied by the downregulation of CDK2 and cyclin E and the upregulation of p21 and p53. As attested by molecular docking study, the dieckol interacted with the core interface residues in transforming growth factor-β receptor with high affinity. These findings suggest that dieckol from E. cava inhibits the cell proliferation of Hs680.Tr, potentially through p21- and p53-mediated G0/G1 cell cycle arrest.
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Dieckol induces cell cycle arrest by down-regulating CDK2/cyclin E in response to p21/p53 activation in human tracheal fibroblasts
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Autor/in / Beteiligte Person: | Yi, Myunggi ; Heo, Seong-Yeong ; Jeong, Min-Seon ; Jung, Won-Kyo ; Won Sun Park ; Hyoung Shin Lee ; Choi, Il-Whan |
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Zeitschrift: | Cell biochemistry and functionREFERENCES, Jg. 40 (2021-09-11), Heft 1 |
Veröffentlichung: | 2021 |
Medientyp: | unknown |
ISSN: | 1099-0844 (print) |
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