Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a Dbl family member
In: FEBS Letters, Jg. 459 (1999-10-12), S. 313-318
Online
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Zugriff:
Several guanine nucleotide exchange factors (GEFs) for Rho-GTPases have been identified, all of them containing a Dbl homology (DH) and pleckstrin homology (PH) domain, but exhibiting different specificities to the Rho family members, Rho, Rac and Cdc42. We report here that KIAA0380, a protein with a tandem DH/PH domain, an amino-terminal PDZ domain and a regulator of G protein signalling (RGS) homology domain, is a specific GEF for RhoA, but not for Rac1 and Cdc42, as determined by GDP release, guanosine 5′-O-(3-thio)triphosphate (GTPγS) binding and protein binding assays. When expressed in J82 cells, DH/PH domain-containing forms of KIAA0380 induced actin stress fibers, whereas expression of the RGS homology domain prevented lysophosphatidic acid (LPA)-induced stress fiber formation.
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Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a Dbl family member
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Autor/in / Beteiligte Person: | Jakobs, Karl ; Schablowski, Helge ; Rümenapp, Ulrich ; Blomquist, Andrea ; Schwörer, Guntram ; Psoma, Amalia |
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Zeitschrift: | FEBS Letters, Jg. 459 (1999-10-12), S. 313-318 |
Veröffentlichung: | Wiley, 1999 |
Medientyp: | unknown |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/s0014-5793(99)01270-3 |
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