Modulation of Balance Between Apoptosis and Proliferation by Lipid Peroxidation (LPO) During Rat Liver Regeneration
In: Molecular Medicine, Jg. 8 (2002-12-01), S. 808-817
Online
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Zugriff:
BACKGROUND: This work aims to investigate the role of lipid peroxidation (LPO) at early stages of liver regeneration and to evaluate the balance between apoptosis and cell proliferation during this process. METHODS: Sham and partial hepatectomized (PH) male Wistar rats were randomized in seven groups: Control (untreated), E-Control (injected with vitamin E-vehicle), C-Control (injected with vitamin C-vehicle), E1 (vitamin E 100 mg/kg body weight), E2 (vitamin E 600 mg/kg body weight), C1 (vitamin C 30 mg/kg body weight), C2 (vitamin C 100 mg/kg body weight). RESULTS: Vitamin treatments attenuated the increase of LPO level observed in total homogenate and microsomes at 3 and 5 hr after PH. Both antioxidant vitamins attenuated the increase in Bax pro-apoptotic protein and augmented Bcl-xL antiapoptotic protein levels (35%) at 3 and 5 hr post-PH; Bcl-xL/Bax ratio was, therefore, increased. A direct linear relationship between LPO levels and Bax mitochondrial protein levels was seen. Vitamin-treatments diminished the apoptosis index with respect to PH-Control values, so that this parameter showed a linear relationship with LPO levels. At 24 hr after PH, the vitamin treatments increased the peak of [(3) H]-thymidine incorporation into DNA and the proliferative index (PI), measured as PCNA expression; an inverse relationship between PI and LPO levels could be demonstrated. CONCLUSION: Our data show that the diminution of LPO levels by vitamin-treatment post-PH produces both an attenuation of cellular apoptosis and a marked increase in the proliferation process, suggesting that the modulation of LPO has a role in liver regeneration process.
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Modulation of Balance Between Apoptosis and Proliferation by Lipid Peroxidation (LPO) During Rat Liver Regeneration
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Autor/in / Beteiligte Person: | Pisani, Gerardo B. ; Monti, Juan A. ; María Cristina Lugano ; María Cristina Carrillo ; María Teresa Ronco ; Carnovale, Cristina E. ; María de Luján Alvarez |
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Zeitschrift: | Molecular Medicine, Jg. 8 (2002-12-01), S. 808-817 |
Veröffentlichung: | Springer Science and Business Media LLC, 2002 |
Medientyp: | unknown |
ISSN: | 1528-3658 (print) ; 1076-1551 (print) |
DOI: | 10.1007/bf03402085 |
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