CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
In: Scientific Reports Scientific Reports, Jg. 7 (2017), Heft 1, S. 1-13
Online
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Zugriff:
Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, faster-growing tumourigenic cells leading to tumours with larger volume and higher mitotic figures. Although CD44br and EpCAMbr cells significantly enriched for tumour-initiating cells (TICs), all cells could retain self-renewal property for at least four generations. Compared to CD44 marker alone, EpCAM/CD44dbr marker did not enhance for cells with faster-growing ability or higher TIC frequency. Cells expressing high CD44 or EpCAM had lower KLF4 and p21 in NPC subpopulations. KLF4-overexpressed EpCAMbr cells had slower growth while Kenpaullone inhibition of KLF4 transcription increased in vitro cell proliferation. Compared to non-NPC, NPC specimens had increased expression of EPCAM, of which tumours from advanced stage of NPC had higher expression. Together, our study provides evidence that EpCAM is a potentially important marker in NPC.
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CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
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Autor/in / Beteiligte Person: | Norazlin Abdul Aziz ; Alan Soo Beng Khoo ; Peh, Suat-Cheng ; Liew, Kitson ; Lu Ping Tan ; Susan Ling Ling Hoe ; Yoon Ming Chin ; Teow, Sin-Yeang ; Cheng Eng Koay ; Tai Lin Chu ; Ching Ching Ng ; Fazlyn Reeny Abdul Razak ; Nurul Ashikin Mohamed Shahrehan ; Kwok Wai Lo ; Noor Kaslina Mohd Kornain ; Ahmad, Munirah |
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Zeitschrift: | Scientific Reports Scientific Reports, Jg. 7 (2017), Heft 1, S. 1-13 |
Veröffentlichung: | Springer Science and Business Media LLC, 2017 |
Medientyp: | unknown |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-017-12045-8 |
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