Neuropeptide Y Inhibits the Biosynthesis of Sulfated Neurosteroids in the Hypothalamus through Activation of Y1Receptors
In: Endocrinology, Jg. 143 (2002-05-01), S. 1950-1963
Online
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Zugriff:
We have recently shown that hydroxysteroid sulfotransferase (HST), the enzyme responsible for the biosynthesis of pregnenolone sulfate (Delta(5)PS) and dehydroepiandrosterone sulfate (DHEAS), is expressed in neurons located in the anterior preoptic area and the dorsal magnocellular nucleus of the frog diencephalon. As these two nuclei are richly innervated by NPY-immunoreactive fibers, we investigated the possible implication of NPY in the control of Delta(5)PS and DHEAS biosynthesis. Double labeling of frog brain sections revealed that 42% of the HST-immunoreactive perikarya in the diencephalon were contacted by NPY-containing fibers. In situ hybridization studies showed that Y(1) and Y(5) receptor mRNAs are expressed in the anterior preoptic area and the dorsal magnocellular nucleus. Pulse-chase experiments with (35)S-labeled 3'-phosphoadenosine 5'-phosphosulfate as a sulfate donor demonstrated that frog NPY (fNPY) inhibited the conversion of [(3)H]Delta(5)P and [(3)H]dehydroepiandrosterone ([(3)H]DHEA) into [(3)H,(35)S]Delta(5)PS and [(3)H,(35)S]DHEAS by diencephalic explants. The inhibitory effect of fNPY on Delta(5)PS and DHEAS formation was mimicked by (pPYY) and [Leu(31),Pro(34)]pNPY, which is an agonist for non-Y(2) receptors in mammals, and was completely suppressed by the Y(1) receptor antagonist BIBP3226. Conversely, the Y(2) receptor agonist pNPY-(13-36) and the Y(5) receptor agonist [D-Trp(32)]pNPY did not significantly modify the biosynthesis of [(3)H,(35)S]Delta(5)PS and [(3)H,(35)S]DHEAS. The present study provides the first evidence for the innervation of neurosteroid-producing neurons by NPY fibers. Our data also demonstrate that NPY, acting via Y(1) receptors, exerts an inhibitory effect on the biosynthesis of sulfated neurosteroids.
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Neuropeptide Y Inhibits the Biosynthesis of Sulfated Neurosteroids in the Hypothalamus through Activation of Y1Receptors
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Autor/in / Beteiligte Person: | Vaudry, Hubert ; Do-Rego, Jean-Luc ; Galas, Ludovic ; Luu-The, Van ; Ayikoe Guy Mensah-Nyagan ; Fournier, Alain ; Fredriksson, Robert ; Beaujean, Delphine ; Larhammar, Dan ; Pelletier, Georges |
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Zeitschrift: | Endocrinology, Jg. 143 (2002-05-01), S. 1950-1963 |
Veröffentlichung: | The Endocrine Society, 2002 |
Medientyp: | unknown |
ISSN: | 1945-7170 (print) ; 0013-7227 (print) |
DOI: | 10.1210/endo.143.5.8765 |
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