Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose
In: Metabolic Engineering Metabolic Engineering, Elsevier, 2016, 38, pp.115-124. ⟨10.1016/j.ymben.2016.07.001⟩ Metabolic Engineering, 2016, 38, pp.115-124. ⟨10.1016/j.ymben.2016.07.001⟩; (2016)
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Zugriff:
Yarrowia lipolytica is a biotechnological chassis for the production of a range of products, such as microbial oils and organic acids. However, it is unable to consume xylose, the major pentose in lignocellulosic hydrolysates, which are considered a preferred carbon source for bioprocesses due to their low cost, wide abundance and high sugar content. Here, we engineered Y. lipolytica to metabolize xylose to produce lipids or citric acid. The overexpression of xylose reductase and xylitol dehydrogenase from Scheffersomyces stipitis were necessary but not sufficient to permit growth. The additional overexpression of the endogenous xylulokinase enabled identical growth as the wild type strain in glucose. This mutant was able to produce up to 80 g/L of citric acid from xylose. Transferring these modifications to a lipid-overproducing strain boosted the production of lipids from xylose. This is the first step towards a consolidated bioprocess to produce chemicals and fuels from lignocellulosic materials.
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Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose
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| Autor/in / Beteiligte Person: | Guo, Zhongpeng ; Anne-Marie Crutz-Le Coq ; Ledesma-Amaro, Rodrigo ; Lazar, Zbigniew ; Rakicka, Magdalena ; Fouchard, Florian ; Nicaud, Jean-Marc ; MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech ; Paris-Saclay, Université ; Department of Biotechnology and Food Microbiology ; Wroclaw University of Environmental and Life Sciences ; Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) ; Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA) ; Université Fédérale Toulouse Midi-Pyrénées ; European Union through the Marie-Curie FP7 COFUND People Program (FP7-267196) ; ANR-11-BTBR-0003 ; Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) ; Université de Toulouse (UT) ; ANR-11-BTBR-0003,PROBIO3,Production biocatalytique de bioproduits lipidiques à partir de matières premières renouvelables et(2011) ; European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012) |
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| Quelle: | Metabolic Engineering Metabolic Engineering, Elsevier, 2016, 38, pp.115-124. ⟨10.1016/j.ymben.2016.07.001⟩ Metabolic Engineering, 2016, 38, pp.115-124. ⟨10.1016/j.ymben.2016.07.001⟩; (2016) |
| Veröffentlichung: | HAL CCSD, 2016 |
| Medientyp: | unknown |
| ISSN: | 1096-7176 (print) ; 1096-7184 (print) |
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