Transcriptome profiling reveals miR-9-3p as a novel tumor suppressor in gastric cancer

// Qingshun Meng 1, * , Longquan Xiang 2, * , Jingwei Fu 3 , Xianqun Chu 3 , Chunlin Wang 3 and Bingzheng Yan 4 1 Department of Gastroenterology, Jining No.1 People’s Hospital, Shandong, China 2 Department of Pathology, Jining No.1 People’s Hospital, Shandong, China 3 Department of Gastrointestinal Surgery, Jining No.1 People’s Hospital, Shandong, China 4 Department of General Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China * These authors have contributed equally to this work Correspondence to: Chunlin Wang, email: chunlin_w001@sina.com Keywords: gastric cancer, microarray, miR-9-3p, prognosis, cell invasion Received: December 28, 2016 Accepted: February 13, 2017 Published: March 17, 2017 ABSTRACT It has been well established that microRNAs (miRNAs) play important roles in biological processes. To comprehensively measure the altered miRNA expression, we presented the miRNA expression profile of gastric cancer using microarray. We identified 33 miRNAs that were significantly differentially regulated in gastric specimens compared to adjacent normal tissues, among which miR-9-3p expression are significantly down-regulated in gastric cancers. Next, a cohort of 100 gastric cancer tissues and matched normal tissues were enrolled. Kaplan–Meier and multivariate Cox survival analyses were applied to evaluate the prognostic value of miR-9-3p expression, and the result showed that patients with lower miR-9-3p expression level have significantly poorer overall survival. The expression level of miR-9-3p has been proved to be an independent prognostic factor for 5-year overall survival. Furthermore, the result indicated that over-expression of miR-9-3p can inhibit gastric cancer cell invasion. Taken together, our results suggested that miR-9-3p plays important role in tumor invasion, and these findings implicated the potential effects of miR-9-3p on prognosis of gastric cancer.