Localized therapy using anti-PD-L1 anchored and NIR-responsive hollow gold nanoshell (HGNS) loaded with doxorubicin (DOX) for the treatment of locally advanced melanoma
In: Nanomedicine : nanotechnology, biology, and medicine, Jg. 33 (2020-06-14)
Online
unknown
Zugriff:
Drug resistance and inefficient localization of chemotherapeutic agent limit the current treatment strategy in locally advanced melanoma (MEL), accounting to the 10-year survival rate from 24% to 68%. In this study we constructed anti-PD-L1 conjugated and doxorubicin loaded hollow gold nanoshell (T-HGNS-DOX) for targeted and localized chemo-photothermal therapy of MEL by the conjugation of LA-PEG-anti-PD-L1 antibody and short PEG chain on the surface of HGNS-DOX. Near infrared (NIR) as well as pH dependent drug release profile was observed. Significant uptake of DOX following NIR due to high PD-L1 receptors resulted in pronounced anticancer effect of T-HGNS-DOX. Following intratumoral administration, maximum nanoparticles retention with the significant reduction in tumor growth was observed as a result of elevated apoptosis marker (cleaved caspase-3, cleaved PARP) as well as downregulation of proliferative (Ki-67) and angiogenesis marker (CD31). Cumulatively, our system avoids the systemic toxicities of the nanosystem thereby providing maximum chemotherapeutic retention in tumor.
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Localized therapy using anti-PD-L1 anchored and NIR-responsive hollow gold nanoshell (HGNS) loaded with doxorubicin (DOX) for the treatment of locally advanced melanoma
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Autor/in / Beteiligte Person: | Banstola, Asmita ; Poudel, Kishwor ; Yook, Simmyung ; Emami, Fakhrossadat ; Jeong, Jee-Heon ; Jong Oh Kim ; Ku, Sae-Kwang |
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Zeitschrift: | Nanomedicine : nanotechnology, biology, and medicine, Jg. 33 (2020-06-14) |
Veröffentlichung: | 2020 |
Medientyp: | unknown |
ISSN: | 1549-9642 (print) |
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