DNA Polymerase I-Dependent Mutants of Coliphage Lambda
In: Journal of Virology, Jg. 13 (1974-05-01), S. 947-952
Online
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Zugriff:
Mutagenized lysates of bacteriophage lambda were screened for mutants unable to plate on DNA polymerase I-deficient ( polA − ) hosts. The mutants obtained were all recombination deficient ( red − ). These mutants, like red − and gam − mutants previously isolated by others, grow more poorly than wild-type λ even on polA + hosts (burst size 14 to 30% of wild-type λ.) In a polA − host, the burst size of red − and gam − mutants is reduced an additional five- to tenfold, and lysis is delayed. Wild-type λ grows normally in polA − hosts. Neither λ N − nin (which doesn't express red or gam ) nor λ bio phages (from which all or part of the red-gam region is deleted) form plaques on polA − hosts. Apparent revertants, able to plate on polA − hosts, have been selected from both λ N − nin and λ bio . Those derived from N − nin seem to be N − nin cro − mutants; whereas those coming from λ bio have a new bypass mutation ( pas ) that lies between genes P and Q .
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DNA Polymerase I-Dependent Mutants of Coliphage Lambda
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Autor/in / Beteiligte Person: | Brown, Douglas L. ; Shizuya, Hiroaki ; Campbell, Allan |
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Zeitschrift: | Journal of Virology, Jg. 13 (1974-05-01), S. 947-952 |
Veröffentlichung: | American Society for Microbiology, 1974 |
Medientyp: | unknown |
ISSN: | 1098-5514 (print) ; 0022-538X (print) |
DOI: | 10.1128/jvi.13.5.947-952.1974 |
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