Protective effects of the suppressed NF‐κB/TLR4 signaling pathway on oxidative stress of lung tissue in rat with acute lung injury
In: The Kaohsiung Journal of Medical Sciences, Jg. 35 (2019-04-18), S. 265-276
Online
unknown
Zugriff:
The pathogenesis of acute lung injury (ALI) is characterized by lung inflammation and lung oxidative stress. The study was conducted in order to investigate the effect toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) exhibited on oxidative stress in ALI. After the rats had been assigned into different groups, arterial blood, white blood cell (WBC), lung permeability index (LPI), wet/dry (W/D) ratio, TLR4 and NF-κB expression and superoxide dismutase (SOD), myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), and reactive oxygen species (ROS) were examined. Afterward, the correlation between the levels of TLR4 and NF-κB was determined. Decreased levels of PaO2 , SOD, MPO, and GSH accompanied by increased levels of PaCO2 , WBC number, LPI and W/D ratio, MDA and ROS, as well as TLR4 and NF-κB expressions in the ALI, ALI + NF-κB inhibitor, and ALI + phosphate buffer saline groups were found. Inhibition of NF-κB resulted in increased PaO2 and decreased PaCO2 levels, WBC number, and LPI and W/D ratio. Decreased expression of NF-κB increased SOD, GSH, and MPO, but decreased MDA and ROS. We also found that NF-κB inhibition resulted in the improvement of ALI in rats. TLR4 and NF-κB expressions were negatively correlated with levels of SOD, MPO, and GSH, and positively correlated with MDA and ROS levels. In summary, our findings provided evidence that inhibition of the TLR4/NF-κB signaling pathway decreases oxidative stress, thereby improving ALI. As a result, NF-κB signaling pathway has shown potential as a therapeutic target in ALI therapy.
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Protective effects of the suppressed NF‐κB/TLR4 signaling pathway on oxidative stress of lung tissue in rat with acute lung injury
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Autor/in / Beteiligte Person: | Zhang, Zeming ; Wang, Yancun ; Li, Zheng ; Chen, Lu |
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Zeitschrift: | The Kaohsiung Journal of Medical Sciences, Jg. 35 (2019-04-18), S. 265-276 |
Veröffentlichung: | Wiley, 2019 |
Medientyp: | unknown |
ISSN: | 2410-8650 (print) ; 1607-551X (print) |
DOI: | 10.1002/kjm2.12065 |
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