Lung Antioxidant Depletion: A Predictive Indicator of Cellular Stress Induced by Ambient Fine Particles
In: Environmental Science & Technology, Jg. 54 (2020-01-21), S. 2360-2369
Online
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Zugriff:
International audience; Regulations on ambient particulate matter (PM) are becoming more stringent because of adverse health effects arising from PM exposure. PM-induced oxidant production is a key mechanism behind the observed health effects and is heavily dependent on PM composition. Measurement of the intrinsic oxidative potential (OP) of PM could provide an integrated indicator of PM bioreactivity and could serve as a better metric of PM hazard exposure than PM mass concentration. The OP of two chemically contrasted PM2.5 samples was compared through four acellular assays, and OP predictive capability was evaluated in different cellular assays on two in vitro lung cell models. PM2.5 collected in Paris at a site close to the traffic exhibited a systematically higher OP in all assays compared to PM2.5 enriched in particles from domestic wood burning. Similar results were obtained for oxidative stress, expression of antioxidant enzymes, and pro-inflammatory chemokine in human bronchial epithelial and endothelial cells. The strongest correlations between OP assays and cellular responses were observed with the antioxidant (ascorbic acid and glutathione) depletion (OPAO) assay. Multivariate regression analysis from OP daily measurements suggested that OPAO was strongly correlated with polycyclic aromatic hydrocarbons at the traffic site while it was correlated with potassium for the domestic wood burning sample.
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Lung Antioxidant Depletion: A Predictive Indicator of Cellular Stress Induced by Ambient Fine Particles
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Autor/in / Beteiligte Person: | Linh Chi Bui ; Baeza-Squiban, Armelle ; Crobeddu, Belinda ; Deweirdt, Juliette ; Sciare, Jean ; Camproux, Anne-Claude ; Baudrimont, Isabelle ; Badel, Anne ; Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) ; Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB) ; Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE) ; Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ) ; Chimie Atmosphérique Expérimentale (CAE) ; Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ) ; Cyprus International Institute for the Environment and Public Health ; Agence de l'Environnement et de la Maitrise de l'Energie, ADEME Agence de la transition écologique, ADEME: 1262C0037, 2012-2-013 ; This work was supported by the French Environment and Energy Management Agency (ADEME: convention no. 1262C0037) in the context of the call for research proposals of the national research program for environmental and occupational health (PNREST) 2012 of ANSES (French Agency for Food, Environmental and Occupational Health & Safety) under grant no. 2012-2-013, project 'POTOX'. We also acknowledge the platform 'FlexStation' and the platform 'Bioprofiler' for HPLC analysis of the facility 'Métabolisme' (Univ de Paris, Unité de Biologie Fonctionnelle et Adaptative (BFA) UMR 8251 CNRS, F-75205, Paris, France), for spectrophotometric analysis. We thank Léa Baudas who helped with OP measurements during her 2 month internship and Oliver Brookes for English proofreading and editing. ; This work was supported by the French Environment and Energy Management Agency (ADEME: convention no. 1262C0037) in the context of the call for research proposals of the national research program for environmental and occupational health (PNREST) 2012 of ANSES (French Agency for Food, Environmental and Occupational Health & Safety) under grant no. 2012-2-013, project 'POTOX'. We also acknowledge the platform 'FlexStation' and the platform 'Bioprofiler' for HPLC analysis of the facility 'Metabolisme' (Univ de Paris, Unite de Biologie Fonctionnelle et Adaptative (BFA) UMR 8251 CNRS, F-75205 Paris, France), for spectrophotometric analysis. We thank Lea Baudas who helped with OP measurements during her 2 month internship and Oliver Brookes for English proofreading and editing. ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) ; Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) ; Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) ; ANSES (French Agency for Food, Environmental and Occupational Health & Safety) under grant no. 2012-2-013, project 'POTOX'. ; Platform 'FlexStation' and the platform 'Bioprofiler' for HPLC analysis of the facility 'Métabolisme' (Univ de Paris, Unité de Biologie Fonctionnelle et Adaptative (BFA) UMR 8251 CNRS, F-75205, Paris, France) |
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Zeitschrift: | Environmental Science & Technology, Jg. 54 (2020-01-21), S. 2360-2369 |
Veröffentlichung: | American Chemical Society (ACS), 2020 |
Medientyp: | unknown |
ISSN: | 1520-5851 (print) ; 0013-936X (print) |
DOI: | 10.1021/acs.est.9b05990 |
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