Polyphenolic profile of persimmon leaves by high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS)

[EN] Persimmon leaves have played an important role in Chinese medicine. Persimmon extracts and formulations have been shown to possess a wide range of pharmacological activities, including antioxidant, hypolipidaemic and antidiabetic, and they have been used to treat cardiovascular disease, improve homeostasis, as antibacterial and anti-inflammatory agents, and as a beauty treatment. In this work, liquid chromatography coupled to hybrid linear ion trap quadrupole Orbitrap mass spectrometry was used to accurately identify persimmon leaf polyphenols. Forty-one phenolic compounds, including simple phenolic acids, hydroxybenzoic acids, hydroxycinnamic acids, flavanols, flavonols, flavanones, flavonechalcones, tyrosols and their conjugated derivatives, were identified and quantified using high mass accuracy data and confirmed by MS2 experiments. To the best of our knowledge, this is the most extensive study of persimmon leaf polyphenols performed so far, since 33 phenolic compounds are reported for the first time. (C) 2016 Elsevier Ltd. All rights reserved.

This work was supported partly by CICYT (AGL2013-49083-C3-1-R) and the Instituto de Salud Carlos III, ISCIII (CIBEROBN) from the Ministerio de Economia y Competitividad (MEC) and Generalitat de Catalunya (GC) 2014 SGR 773. Paola Quifer-Rada is grateful for the predoctoral fellowships awarded by the Generalitat de Catalunya (FI-DRG). Also, the authors acknowledge the support of the Universitat Politecnica de Valencia and certify that there is no conflict of interest with any financial organisation regarding the material discussed in the manuscript.