A Major Portion of DNA Gyrase Inhibitor Microcin B17 Undergoes an N,O-Peptidyl Shift during Synthesis
In: Journal of Biological Chemistry, Jg. 286 (2011-07-01), S. 26308-26318
Online
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Zugriff:
Microcin B17 (McB) is a 43-amino acid antibacterial peptide targeting the DNA gyrase. The McB precursor is ribosomally produced and then post-translationally modified by the McbBCD synthase. Active mature McB contains eight oxazole and thiazole heterocycles. Here, we show that a major portion of mature McB contains an additional unusual modification, a backbone ester bond connecting McB residues 51 and 52. The modification results from an N → O shift of the Ser(52) residue located immediately downstream of one of McB thiazole heterocycles. We speculate that the N,O-peptidyl shift undergone by Ser(52) is an intermediate of post-translational modification reactions catalyzed by the McbBCD synthase that normally lead to formation of McB heterocycles.
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A Major Portion of DNA Gyrase Inhibitor Microcin B17 Undergoes an N,O-Peptidyl Shift during Synthesis
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Autor/in / Beteiligte Person: | Serebryakova, Marina V. ; Ghilarov, Dmitry ; Severinov, Konstantin ; Shkundina, Irina |
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Zeitschrift: | Journal of Biological Chemistry, Jg. 286 (2011-07-01), S. 26308-26318 |
Veröffentlichung: | Elsevier BV, 2011 |
Medientyp: | unknown |
ISSN: | 0021-9258 (print) |
DOI: | 10.1074/jbc.m111.241315 |
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