Effect of Two Lipid Emulsions on Reversing High-Dose Levobupivacaine-Induced Reduced Vasoconstriction in the Rat Aortas
In: Cardiovascular Toxicology, Jg. 13 (2013-07-23), S. 370-380
Online
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Zugriff:
The goals of this study were to determine which lipid emulsion (Intralipid(®) and Lipofundin MCT/LCT(®)) is more effective in reversing high-dose levobupivacaine-induced reduced vasoconstriction in isolated rat aortas and to examine the associated cellular mechanisms with a particular focus on the endothelium. Two lipid emulsion concentration-response curves were generated using high-dose levobupivacaine-induced reduced vasoconstriction and vasodilation of isolated aortas pretreated with or without 60 mM KCl. Endothelial nitric oxide synthase (eNOS) and caveolin-1 phosphorylation were measured in rat aortic tissue treated with levobupivacaine in the presence or absence of lipid emulsion. Dichlorofluorescein oxidation, a measure of reactive oxygen species production, was measured in lipid emulsion-treated human umbilical vein endothelial cells. In levobupivacaine (0.3 mM)-induced reduced vasoconstriction of isolated aorta, the magnitude of the Intralipid(®)- and Lipofundin MCT/LCT(®)-mediated reversal was not significantly different. Lipid emulsion reversal of levobupivacaine-induced reduced vasoconstriction was greater in endothelium-intact aortas than in endothelium-denuded aortas. The two lipid emulsions similarly inhibited levobupivacaine-induced eNOS phosphorylation in aortic tissue. Pretreatment with both lipid emulsions increased dichlorofluorescein oxidation. Both Intralipid(®) and Lipofundin MCT/LCT(®) are equally effective for vascular tone recovery from high-dose levobupivacaine-induced reduced vasoconstriction. This reversal is mediated partially by decreasing nitric oxide bioavailability.
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Effect of Two Lipid Emulsions on Reversing High-Dose Levobupivacaine-Induced Reduced Vasoconstriction in the Rat Aortas
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Autor/in / Beteiligte Person: | Soo Hee Lee ; Kim, Kyung-Nam ; So Young Eun ; Seong Min Yang ; Park, Chang-Shin ; Choi, Bo-Hwa ; Ok, Seong-Ho ; Choi, Mun-Jeoung ; Il Woo Shin ; Sohn, Ju-Tae ; Hye Jung Kim |
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Zeitschrift: | Cardiovascular Toxicology, Jg. 13 (2013-07-23), S. 370-380 |
Veröffentlichung: | Springer Science and Business Media LLC, 2013 |
Medientyp: | unknown |
ISSN: | 1559-0259 (print) ; 1530-7905 (print) |
DOI: | 10.1007/s12012-013-9218-y |
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