Inhibition of poly(ADP-ribose)polymerase stimulates extrachromosomal homologous recombination in mouse Ltk- fibroblasts
In: Nucleic Acids Research, Jg. 27 (1999-11-01), S. 4526-4531
Online
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Zugriff:
Poly(ADP-ribose)polymerase (PARP) is an abundant nuclear enzyme activated by DNA breaks. PARP is generally believed to play a role in maintaining the integrity of the genome in eukaryote cells via anti-recombinogenic activity by preventing inappropriate homologous recombination reactions at DNA double-strand breaks. While inhibition of PARP reduces non-homologous recombination, at the same time it stimulates sister chromatid exchange and intrachromosomal homologous recombination. Here we report that the inhibition of PARP with 100 microg/ml (0.622 mM) 1,5-isoquinolinediol results in an average 4.6-fold increase in the frequency of extrachromosomal homologous recombination between two linearized plasmids carrying herpes simplex virus thymidine kinase genes inactivated by non-overlapping mutations, in mouse Ltk-fibroblasts. These results are in disagreement with the previously reported observation that PARP inhibition had no effect on extrachromosomal homologous recombination in Ltk-cells.
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Inhibition of poly(ADP-ribose)polymerase stimulates extrachromosomal homologous recombination in mouse Ltk- fibroblasts
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Autor/in / Beteiligte Person: | Cournoyer, Denis ; Terry Y.-K. Chow ; Semionov, Alexandre |
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Zeitschrift: | Nucleic Acids Research, Jg. 27 (1999-11-01), S. 4526-4531 |
Veröffentlichung: | Oxford University Press (OUP), 1999 |
Medientyp: | unknown |
ISSN: | 1362-4962 (print) ; 0305-1048 (print) |
DOI: | 10.1093/nar/27.22.4526 |
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