Effects of the anti-androgen cyproterone acetate (CPA) on oocyte meiotic maturation in rainbow trout [i](Oncorhynchus mykiss)[/i]
In: Aquatic Toxicology Aquatic Toxicology, Elsevier, 2015, 164, pp.34-42. ⟨10.1016/j.aquatox.2015.04.009⟩; (2015)
Online
unknown
Zugriff:
In the present study, we aimed at characterizing the effect of cyproterone acetate (CPA), an anti-androgenic compound, on oocyte meiotic maturation in a freshwater teleost fish species, the rainbow trout [i](Oncorhynchus mykiss)[/i]. Fully-grown post-vitellogenic ovarian follicles were incubated in vitro with CPA, luteinizing hormone (Lh) or a combination of CPA and Lh. Incubations were also performed using a combination of Lh and testosterone (T). The occurrence of oocyte maturation (i.e., resumption of the meiotic process) was assessed by monitoring germinal vesicle breakdown (GVBD) after a 72 h[i] in vitro[/i] incubation. The effect of CPA on the production of 17,20β-dihydroxy-4-pregnen-3-one (17,20βP), the natural maturation-inducing steroid (MIS), was quantified by radioimmunoassay. Our results show that CPA dramatically inhibits Lh-induced oocyte maturation and MIS synthesis. We also observed a synergistic effect of Lh and T on oocyte maturation in highly competent oocytes (i.e., able to resume meiosis after stimulation by low doses of Lh). Our results also show that a combination of CPA and Lh inhibits phosphorylation of extracellular signal-regulated kinase (Erk), kinases that are associated with oocyte maturation in many species. As a whole, our results indicate that CPA has a potential to alter meiotic maturation in rainbow trout. Further analyses are, however, needed to determine the mechanisms by which this anti-androgen interferes with the meiotic process. Furthermore, the present study provides a framework for better understanding of the ecological consequences of exposure to anti-androgens and resulting meiotic maturation abnormalities observed in trout.
Titel: |
Effects of the anti-androgen cyproterone acetate (CPA) on oocyte meiotic maturation in rainbow trout [i](Oncorhynchus mykiss)[/i]
|
---|---|
Autor/in / Beteiligte Person: | Bobe, Julien ; Rime, Hélène ; Thuy Thao Vi Nguyen ; Ombredane, Kévin ; Fostier, Alexis ; Laboratoire de Physiologie et Génomique des Poissons (LPGP) ; Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
Link: | |
Quelle: | Aquatic Toxicology Aquatic Toxicology, Elsevier, 2015, 164, pp.34-42. ⟨10.1016/j.aquatox.2015.04.009⟩; (2015) |
Veröffentlichung: | HAL CCSD, 2015 |
Medientyp: | unknown |
ISSN: | 0166-445X (print) |
Schlagwort: |
|
Sonstiges: |
|