CRISPR/dCas-mediated transcriptional and epigenetic regulation in plants
In: Current Opinion in Plant Biology, Jg. 60 (2021-04-01), S. 101980-101980
Online
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Zugriff:
The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR Associated) system-mediated precise genome editing has revolutionized genome engineering due to ease of use and versatility of multiplexing. Catalytically inactivated Cas variants (dCas) further expand the usefulness of the CRISPR/Cas system for genetics studies and translational research without inducing DNA double-strand breaks. Fusion of diverse effector domains to dCas proteins empowers the CRISPR/dCas system as a multifunctional platform for gene expression regulation, epigenetic regulation and sequence-specific imaging. In this short review, we summarize the recent advances of CRISPR/dCas-mediated transcriptional activation and repression, and epigenetic modifications. We also highlight the future directions and broader applications of the CRISPR/dCas systems in plants.
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CRISPR/dCas-mediated transcriptional and epigenetic regulation in plants
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Autor/in / Beteiligte Person: | Sretenovic, Simon ; Qi, Yiping ; Pan, Changtian |
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Zeitschrift: | Current Opinion in Plant Biology, Jg. 60 (2021-04-01), S. 101980-101980 |
Veröffentlichung: | Elsevier BV, 2021 |
Medientyp: | unknown |
ISSN: | 1369-5266 (print) |
DOI: | 10.1016/j.pbi.2020.101980 |
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