Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway
In: Cell Biology and Toxicology, Jg. 36 (2020-04-11), S. 493-507
Online
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Zugriff:
A percentage of colorectal cancer (CRC) patients display low sensitivity to radiotherapy, which affects its therapeutic effect. Cancer cells DNA double-strand breaks (DSBs) repair capacity is crucial for radiosensitivity, but the roles of long noncoding RNAs (lncRNAs) in this process are largely uncharacterized. This study aims to explore whether lnc-RI regulates CRC cell growth and radiosensitivity by regulating the nonhomologous end-joining (NHEJ) repair pathway. CRC cells in which lnc-RI has been silenced showed lower cell growth and higher apoptosis rates due to increased DSBs and cell cycle arrest. We found that miR-4727-5p targets both lnc-RI and LIG4 mRNA and inhibit their expression. CRC cells showed increased radiosensitivity when lnc-RI was silenced. These results reveal novel roles for lnc-RI in both DNA damage repair and radiosensitivity regulation in CRC cells. Our study revealed that lnc-RI regulates LIG4 expression through lnc-RI/miR-4727-5p/LIG4 axis and regulates NHEJ repair efficiency to participate in DNA damage repair. The level of lnc-RI was negatively correlated with the radiosensitivity of CRC cells, indicates that lnc-RI may be a potential target for CRC therapy. We also present the first report of the function of miR-4727-5p.
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Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway
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Autor/in / Beteiligte Person: | Qi, Zhenhua ; Zhang, Qingtong ; Liu, Ruixue ; Wang, Dong ; Chen, Shuangjing ; Shen, Liping ; Wang, Zhidong ; Qi, Wang ; Zhou, Meijuan ; He, Junyan |
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Zeitschrift: | Cell Biology and Toxicology, Jg. 36 (2020-04-11), S. 493-507 |
Veröffentlichung: | Springer Science and Business Media LLC, 2020 |
Medientyp: | unknown |
ISSN: | 1573-6822 (print) ; 0742-2091 (print) |
DOI: | 10.1007/s10565-020-09524-6 |
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