Measurement of Molt-inhibiting Hormone Titer in Hemolymph of the American Crayfish, Procambarus clarkii, by Time-Resolved Fluoroimmunoassay
In: Zoological Science, Jg. 20 (2003-08-01), S. 999-1001
Online
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Zugriff:
In order to determine the titer of molt-inhibiting hormone (Prc-MIH) in the hemolymph of the American crayfish Procambarus clarkii, a time-resolved fluoroimmunoassay (TR-FIA) was established using specific antibodies against N-terminal and C-terminal segments of Prc-MIH. The lowest limit of detection of Prc-MIH in TR-FIA was 10 amol/assay. The Prc-MIH titers in the hemolymph were 6.53 fmol/ml at the intermolt stage and 1.25 fmol/ml at the early premolt stage. This result is consistent with the long-known hypothesis that the Y-organ is inhibited by MIH during the intermolt stage, whereas the Y-organ is activated by being freed from the inhibitory regulation of MIH.
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Measurement of Molt-inhibiting Hormone Titer in Hemolymph of the American Crayfish, Procambarus clarkii, by Time-Resolved Fluoroimmunoassay
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Autor/in / Beteiligte Person: | Sonobe, Haruyuki ; Nakatsuji, Teruaki |
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Zeitschrift: | Zoological Science, Jg. 20 (2003-08-01), S. 999-1001 |
Veröffentlichung: | Zoological Society of Japan, 2003 |
Medientyp: | unknown |
ISSN: | 0289-0003 (print) |
DOI: | 10.2108/zsj.20.999 |
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