Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan
In: Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Jg. 11 (2021), Heft 6, S. 717-725
Online
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Zugriff:
Xiaoer-Feire-Kechuan (XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation. The complicated composition rendered its chemical analysis and effective-component elucidation. In this study, we combined quantitative analysis and bioactivity test to reveal the anti-inflammatory constituents of XFK. First, UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes (covering 9 out of 11 herbs) in different XFK formulations. Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity. Then, anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay. Finally, major analytes forsythosides H, I, A (8–10), and baicalin (15) (total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities (inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK. The present study provided an effective strategy to discover effective constituents of multi-herb formulas.
Graphical abstract Image 1
Highlights ∙ Effective components were elucidated for XFK, a 11-herb formula. ∙Contents of 35 compounds were determined using UPLC-DAD and UHPLC/Q-Orbitrap-MS. ∙Parallel Reaction Monitoring mode was used to improve the sensitivity. ∙COX-2 inhibition assay was used to evaluate the bioactivity of 35 compounds. ∙Five major compounds were proposed as anti-inflammatory constituents for XFK.
Titel: |
Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan
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Autor/in / Beteiligte Person: | Liu, Shuang ; Lin, Yan ; Qiao, Xue ; Bo, Tao ; Sun, Long ; Ye, Min ; Kuang, Yi ; Shang, Zhanpeng ; Xu, Lulu |
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Zeitschrift: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Jg. 11 (2021), Heft 6, S. 717-725 |
Veröffentlichung: | Elsevier BV, 2021 |
Medientyp: | unknown |
ISSN: | 2095-1779 (print) |
DOI: | 10.1016/j.jpha.2021.01.003 |
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