Three NPF genes in Arabidopsis are necessary for normal nitrogen cycling under low nitrogen stress
In: Plant Physiology and Biochemistry, Jg. 143 (2019-10-01), S. 1-10
Online
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Zugriff:
Internal nitrogen (N) cycling is crucial to N use efficiency. For example, N may be remobilized from older, shaded leaves to young leaves near the apex that receive more direct sunlight, where the N can be used more effectively for photosynthesis. Yet our understanding of the mechanisms and regulation of N transport is limited. To identify relevant transporters in Arabidopsis, fifteen transporter knockout mutants were screened for defects in leaf N export using nitrogen-13 (13N) administered as 13NH3 gas to leaves. We found that three nitrate/peptide transporter family (NPF) genes were necessary for normal leaf N export under low N but not adequate soil N availability, including AtNPF7.1, which has not been previously characterized. High-throughput phenotyping revealed altered leaf area and chlorophyll fluorescence relative to wild-type plants. High AtNPF7.1 expression in flowers and large flower stalks of Atnpf7.1 mutants in low N suggests that AtNPF7.1 influences leaf N export via sink-to-source feedback, perhaps via a role in sensing plant internal N-status. We also identified previously unreported phenotypes for the mutants of the other two NPF transporters that indicate possible roles in N sensing networks.
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Three NPF genes in Arabidopsis are necessary for normal nitrogen cycling under low nitrogen stress
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Autor/in / Beteiligte Person: | Lorence, Argelia ; Schueller, Michael J. ; Gao, Fei ; Acosta-Gamboa, Lucia M. ; Babst, Benjamin A. ; Karve, Abhijit |
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Zeitschrift: | Plant Physiology and Biochemistry, Jg. 143 (2019-10-01), S. 1-10 |
Veröffentlichung: | Elsevier BV, 2019 |
Medientyp: | unknown |
ISSN: | 0981-9428 (print) |
DOI: | 10.1016/j.plaphy.2019.08.014 |
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