Identification of an invasion-inducing gene, Tiam-1, that encodes a protein with homology to GDP-GTP exchangers for Rho-like proteins
In: Cell, Jg. 77 (1994-05-20), Heft 4
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Zugriff:
Using proviral tagging in combination with in vitro selection for invasiveness, we have identified a gene, designated Tiam-1 , that affects invasion. In the selected invasive T lymphoma variants, proviral insertions were found within coding exons of the Tiam-1 gene, resulting in both truncated 5′-end and 3′-end transcripts that give rise to N- and C-terminal Tiam-1 protein fragments. In one invasive variant, amplification of the Tiam-1 locus was observed with concomitant increase in the amount of normal Tiam-1 protein. Cell clones that were invasive in vitro produced experimental metastases in nude mice, and transfection of truncated Tiam-1 cDNAs into noninvasive cells made these cells invasive. The predicted Tiam-1 protein harbors a Dbl- and Pleckstrin-homologous domain, which it shares with GDP-GTP exchangers for Rho-like proteins that have been implicated in cytoskeletal organization.
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Identification of an invasion-inducing gene, Tiam-1, that encodes a protein with homology to GDP-GTP exchangers for Rho-like proteins
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Autor/in / Beteiligte Person: | Collard, John G. ; Stam, Jord C. ; Zuydgeest, David ; Berns, Anton ; Habets, G. G. M. ; Ellen H.M. Scholtes ; Rob A. van der Kammen |
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Zeitschrift: | Cell, Jg. 77 (1994-05-20), Heft 4 |
Veröffentlichung: | 1994 |
Medientyp: | unknown |
ISSN: | 0092-8674 (print) |
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