Indoor fungal contamination of moisture-damaged and allergic patient housing analysed using real-time PCR
In: Letters in Applied Microbiology Letters in Applied Microbiology, Wiley, 2009, 49 (2), pp.260-6. 〈10.1111/j.1472-765X.2009.02653.x〉 Letters in Applied Microbiology, Wiley, 2009, 49 (2), pp.260-6. ⟨10.1111/j.1472-765X.2009.02653.x⟩; (2009-08-01)
Online
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Zugriff:
International audience; AIMS: The aim of our study was to compare, using real-time (Rt) PCR, quantitative levels of five fungal species in three kinds of dwellings. METHODS AND RESULTS: Three groups of homes were recruited: moisture-damaged homes (MDH, n = 30), allergic patient homes (APH, n = 25) and paired control homes (CH, n = 55). Five moulds with allergenic compounds or mycotoxin production characteristics (Cladosporium sphaerospermum, Penicillium chrysogenum, Aspergillus versicolor, Alternaria alternata and Stachybotrys chartarum) were quantified using Rt-PCR. Cycle threshold results were expressed in spore equivalent per volume or surface unit using a direct calculation based on a spore standard curve. MDH presented significantly higher amounts of DNA from C. sphaerospermum in both air and surface samples than CH (P < 0.001). APH presented slightly elevated amounts of DNA from A. versicolor in both air and surface samples, compared to CH (P < 0.05). CONCLUSION: Rt-PCR quantification of targeted fungal species is a rapid, reliable tool that could be included in a global indoor mould evaluation. SIGNIFICANCE AND IMPACT OF THE STUDY: Quantification of C. sphaerospermum using Rt-PCR can help to better target social service intervention in MDH. Quantification of A. versicolor DNA could be informative for characterization of APH.
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Indoor fungal contamination of moisture-damaged and allergic patient housing analysed using real-time PCR
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Autor/in / Beteiligte Person: | Millon, Laurence ; Didier-Scherer, E. ; Dalphin, Jean-Charles ; Roussel, Sandrine ; Reboux, Gabriel ; Grenouillet, Frédéric ; Bellanger, Anne-Pauline ; Laboratoire Chrono-environnement ( LCE ) ; Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ) ; Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE) ; Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) ; Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC) |
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Quelle: | Letters in Applied Microbiology Letters in Applied Microbiology, Wiley, 2009, 49 (2), pp.260-6. 〈10.1111/j.1472-765X.2009.02653.x〉 Letters in Applied Microbiology, Wiley, 2009, 49 (2), pp.260-6. ⟨10.1111/j.1472-765X.2009.02653.x⟩; (2009-08-01) |
Veröffentlichung: | HAL CCSD, 2009 |
Medientyp: | unknown |
ISSN: | 0266-8254 (print) ; 1472-765X (print) |
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